Journal of Neurology Research, ISSN 1923-2845 print, 1923-2853 online, Open Access
Article copyright, the authors; Journal compilation copyright, J Neurol Res and Elmer Press Inc
Journal website http://www.neurores.org

Original Article

Volume 4, Number 1, February 2014, pages 7-14

Color-Coded Microspheres and Histological Analysis for Cerebral Mapping: An Experimental Model

Figures

Figure 1.
Figure 1. (a) Brain slicing model with axial cutting. (b) Antero-posterior slicing of 5 mm sections throughout the brain in a sagital view.
Figure 2.
Figure 2. Confidence interval of RMBF over time points.
Figure 3.
Figure 3. Confidence interval of RMBF over brain slices.
Figure 4.
Figure 4. Confidence interval of RMBF among subjects.
Figure 5.
Figure 5. (a) Representation of H&E staining of a normal brain slice. Slice corresponding to frontal lobes at the deep gray matter including “pencil” white matter tracts. (b) Same slice after APP staining with negative APP immunohistochemical staining indicating no axonal injury.
Figure 6.
Figure 6. (a) H&E of brain slice at the fronto-parietal region showing a needle track cavity with fragments of necrotic brain and surrounding penumbra of pale edematous brain. (b) Same slice after APP staining highlighting axonal injury around the necrotic needle track.
Figure 7.
Figure 7. Brain slice at the parietal region showing two clear refractile microspheres trapped in a small cerebral vessel that runs diagonally across the image. The surrounding gray matter appears normal (H&E stain × 400 magnification).

Table

Table 1. RMBF Means (Standard Deviations) by Each Brain Slice Over Time (T), Expressed in mL/min/g
 
SliceT0T1T2T3T4
Heart0.05 (NA)0.06 (NA)0.05 (NA)0.02 (NA)0.07 (NA)
Skin2.70 (NA)1.65 (NA)2.38 (NA)1.26 (NA)4.07 (NA)
Medulla1.06 (0.71)0.74 (0.57)1.92 (2.06)4.83 (6.54)0.66 (NA)
Midbrain0.45 (0.24)0.58 (0.35)0.55 (0.42)0.76 (0.74)0.63 (0.33)
Pons0.64 (0.21)0.99 (0.90)0.86 (0.83)2.28 (3.20)0.79 (0.27)
Slice 020.57 (0.26)1.02 (1.20)0.89 (1.13)NA (NA)0.71 (0.47)
Slice 040.45 (0.15)0.71 (0.59)0.76 (0.88)1.42 (1.70)1.04 (1.11)
Slice 06NA (NA)0.67 (0.55)0.72 (0.82)1.87 (3.00)0.35 (0.33)
Slice 080.52 (0.30)0.63 (0.41)0.46 (0.33)1.77 (2.85)1.46 (1.93)
Slice 100.42 (0.16)0.45 (0.27)0.34 (0.21)1.28 (2.12)0.38 (0.33)
Slice 120.66 (0.26)0.75 (0.67)0.35 (0.35)1.58 (2.28)0.86 (0.67)
Slice 140.48 (0.13)0.67 (0.58)0.31 (0.30)1.15 (1.58)0.29 (0.29)